Wyberton lost 6-1 at Hykeham Town in the Challenge Cup.
Ben Jaques netted the consolation goal.
Gary Frost’s side currently sit third in the Lincolnshire League, with three games in hand on second-placed Cleethorpes Town DS, who have a two-point advantage.
Unbeaten Sleaford Town Reserves remain top of the table.
They have also played three games more than Wyberton, but have a 12-point cushion.
On Saturday they return to league action as they host Horncastle Town at the Causeway (KO 2pm).